TransforMax™ EC100™ Electrocompetent E. coli TransforMax™ EC100™感受态大肠杆菌
货号：EC10010
规格：20 rxns
价格：3824
产品类型：感受态细胞
品牌：Lucigen
| TransforMax™ EC100™ Electrocompetent E. coli are ideal for all cloning applications. Their higher efficiency, lack of size bias and other features make them ideal for transformation of EZ-Tn5™ Transposon insertion clones. Since TransforMax EC100 cells are restriction minus and lack transformation size bias against large inserts, they can be used to generate complete and unbiased primary bacterial artificial chromosome (BAC) libraries. |
| 优点： |
| ▪ Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.▪ Achieve high efficiencies with convenient chemically competent format: >5 × 108 cfu/µg pUC19 DNA.▪ Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations. |
| 相关产品： |
| ▪ EZ-Tn5<T7/KAN-2>Promoter Insertion Kits：(# EZI03T7）▪ EZ-Tn5<DHFR-1>Insertion Kit ：(# EZI912D）▪ EZ-Tn5<KAN-2> Insertion Kit：(# EZI982K）▪ EZ-Tn5<TET-1> Insertion Kit ：(# EZI921T） |
| 参考文献： |
| 1. Metcalf, W.W. et al., (1994) Gene 138, 1. 2. Hanahan, D. (1983) J. Mol. Biol., 166, 557. |
技术参数 产品优点 - Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA. - Achieve high efficiencies with convenient chemically competent format: >5 × 108 cfu/µg pUC19 DNA. - Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations.
产品应用 - Ultra-high transformation efficiency (>1 × 1010 cfu/μg of supercoiled DNA). - Compatible with vectors expressing the LacZ′ α-complementing peptide for “blue/white” screening of recombinants. - Restriction minus for efficient cloning of methylated genomic DNA. - Accepts large clones for unbiased, primary cosmid and BAC clone library production. - Endonuclease minus (endA1) to ensure high yields of plasmid clones. - Recombination minus (recA1) to ensure the stability of large cloned inserts.
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