TransforMax™ EC100™ Chemically Competent E. coli TransforMax™ EC100™化学感受态大肠杆菌
货号:CC02810
规格:10 rxns
价格:1744
产品类型:感受态细胞
品牌:Lucigen
| TransforMax™ EC100™ Chemically Competent E. coli are useful for primary cloning as well as subcloning applications. The cells are provided in 50 μl aliquots (1 transformation per tube) for ease of use. The transformation efficiency of the TransforMax EC100 Chemically Competent cells is >1 × 108 cfu/μg DNA using pUC19.Relevant Phenotype • Compatible with vectors expressing the LacZ′α-complementing peptide for “blue/white” screening of recombinants. • Restriction minus for efficient cloning of methylated DNA. • Accepts large clones. • Endonuclease minus (endA1) to ensure high yields of plasmid clones.• Recombination minus (recA1) to ensure the stability of large cloned inserts. |
| 优点: |
| ▪ Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.▪ Achieve high efficiencies with convenient chemically competent format: >5 × 108 cfu/µg pUC19 DNA.▪ Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations. |
| 组成成分: |
| ▪ TransforMax™ EC100™ Chemically Competent E. coli includes: 10 tubes of cells:-70℃▪ pUC19 Control DNA (100 pg/μl):-70℃ |
技术参数 产品优点 - Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA. - Achieve high efficiencies with convenient chemically competent format: >5 × 108 cfu/µg pUC19 DNA. - Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations.