CopyCutter™ EPI400™ Chemically Competent E. coli/ CopyCutter™ EPI400™化学感受态大肠杆菌
货号：C400CH10
规格：10 rxns
价格：2455
产品类型：感受态细胞
品牌：Lucigen
| CopyCutter™ EPI400™ Electrocompetent and Chemically Competent E. coli cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. Moreover, following a short incubation in the presence of the CopyCutter Induction Solution, you can subsequently raise copy number to improve plasmid yields without compromising stability. The CopyCutter EPI400 cell line was derived from our high-transformation efficiency phage T1-resistant TransforMax™ EC100™ E. coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC and pET type vectors). This constitutively expressed gene, pcnB (plasmid copy number), was deleted from the TransforMax EC100 strain and replaced with a modified pcnB gene linked to an inducible promoter, creating the CopyCutter EPI400 strain. |
| 优点： |
| ▪ High transformation efficiency with clones of all sizes. ▪ Supports blue/white screening of vectors. ▪ Restriction minus [mcrA ∆(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated DNA. ▪ Endonuclease minus (endA1) to ensure high yields of plasmid clones. ▪ Recombination minus (recA1) to ensure the stability of large cloned inserts. |
产品优点 - High transformation efficiency with clones of all sizes. - Supports blue/white screening of vectors. - Restriction minus [mcrA ∆(mrr-hsdRMS-mcrBC)] for efficient cloning of methylated DNA. - Endonuclease minus (endA1) to ensure high yields of plasmid clones. - Recombination minus (recA1) to ensure the stability of large cloned inserts.
产品应用 - Stabilize toxic inserts in common cloning and expression vectors (pUC and pET-type vectors) - Clone and maintain challenging sequences at reduced plasmid copy number, then induce to high copy number for DNA recovery - Avoid T1 and T5 phage contamination with tonA mutation - Choose chemically competent cells for general cloning or electrocompetent cells for demanding applications such as library generation
QQ:1749072012