AFP Monoclonal Antibody (F1-6P2A8-P2B9A9)
货号： MA5-14665
规格：100 µg
价格：5467
产品类型：免疫组化一抗
品牌：eBioscience
物种：人
宿主：小鼠
抗体亚型：IgG
荧光染料：Purified
类型: | 单抗 | 同型对照： | |
浓度： | 1 mg/mL | 用法： | 5 µg/mL (ICC)；5 µg/mL (IF)；Assay Dependent（IP）；1 µg/mL(WB) ；Assay Dependent （RIA）；Assay Dependent （ELISA） |
免疫原性： | Purified AFP from Cord Blood |
产品详细信息
MA5-14665 can be used for immunofluorescent analysis of AFP in the endoderm derived from human embryonic stem cells. By Western blot, MA5-14665 detects endogenous AFP protein in the early hepatocyte-like cells derived from human embryonic stem cells.Product MA514665 is a smaller package size of MIA1301 (formerly sold as a Seradyn product).
靶标信息
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
数据 |
| AFP Antibody (MA5-14665) in IF Immunofluorescence analysis of alpha-fetoproprotein (AFP) (green) in the endoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09) using Gibco® KnockOut™ Serum Replacement. After four days in suspension culture, EBs were plated on Geltrex™-coated tissue culture-treated polystyrene plates and continuously cultured for 21 days. EB cultures were then fixed and permeabilized according to the 3-Germ Layer Immunocytochemistry Kit (Product # A25538) and stained with anti-alpha-fetoprotein (Product # MA5-14665, 1:200 dilution, 5 uL/mL final) at 4°C overnight. Secondary staining was completed using Alexa Fluor™ 488-conjugated anti-mouse IgG (Product # A-11001) and DAPI (Product # D1306) for nuclear DNA (blue) for 1 h at room temperature. Stained wells were imaged at 40X using the EVOS® FL Auto Imaging System. AFP Antibody (MA5-14665) in WBWestern blot analysis of alpha-fetoprotein was performed by loading 10 µg of whole cell extract from H9 ESCs, primary human hepatocytes, HepaRG cells, HepG2 cells, and H9 ESC-derived hepatocytes-like cells in reducing conditions and 10 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) per well onto a Novex Bolt® 4-12% Bis-Tris Plus Gel with Bolt® MOPS running buffer and Bolt® Antioxidant. Proteins were transferred to a PVDF membrane using the iBlot 2 Dry Blotting System (Product # IB21001), and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. alpha-Fetoprotein was detected at 67 kDa using an alpha-fetoprotein monoclonal antibody (Product # MA5-14665) at a concentration of 1 µg/mL in 5% non-fat milk in TBST overnight at 4°C on a rocking platform, followed by a goat anti-mouse secondary antibody (Product # A24518) at a dilution of 1:10,000 for 1 hour at room temperature. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
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