Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit 小鼠IgG1 Fab和F(ab')2制备试剂盒

2024-10-17

Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit 小鼠IgG1 Fab和F(ab')2制备试剂盒

货号:44980

规格:1 kit

价格:10426

产品类型:蛋白提取/纯化

品牌:Thermo Fisher

The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.
特点:
- High capacity—kit provides for fragmenting 0.25 to 4 mg IgG.- Complete—kit includes all reagents needed to prepare and purify antibody fragments- Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme- Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass
数据:

Depending on the concentration of cysteine used in the digestion buffer, primarily F(ab')2 or Fab can be made.

Analysis of various experimental fractions by non-reducing SDS-PAGE (8-16%). Each well was loaded with 2µL of protein.

技术参数
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