BHQ-3 CPG; Glycolate, 1000 Å
货号:BG1-5043G-1,BG1-5043G-100
规格:1 g,100 mg
价格:23216,2941
产品类型:核酸合成
品牌:LGC Biosearch Technologies
| BHQ®-3 Glycolate CPG is used to add a non-fluorescent quencher (BHQ-3 dye) at the 3' end of an oligonucleotide and has been designed to have maximal absorption in the 620 nm to 730 nm range, which provides excellent quenching of fluorophores emitting in this range, e.g. CAL Fluor® Red 635, Cy5, Cy5.5, Pulsar® 650 and Quasar® 670 dyes. This product has a glycolate linkage to the CPG support which allows for rapid cleavage of the oligonucleotide and is labile enough for base sensitive oligonucleotides. The use of fast deprotecting amidites is recommended. |
| 特性: |
| - Appearance: dark blue powder- Absorption Maximum (Lambda Max): 672- Extinction Coefficient at Lambda max:42700- Extinction Coefficient at 260 nm:13000 |
| 产品用途: |
| - Synthesis conditions: We recommend only using fast deprotecting amidites with this product due to the mild cleavage and deprotection conditions.- Deprotection conditions: Cleave and deprotect in concentrated NH4OH for 1 hour at 60 °C (after 1 hour at 60 °C, degradation of product may occur).- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:597.63 |
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