Universal Support-CPG (DMT-Ribose-Linker); 500 Å
货号：BG5-3400-1
规格：1 g
价格：844
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Oligonucleotide synthesis using Universal Support CPG is performed using standard synthesis protocols for 1.0 µmol, 200 nmol or 50 nmol scale. The CPG support does not contribute to the base sequence; therefore, it may be necessary to include a nonsense base as the 3' terminal base for sequence entry systems. The 500 Å pore size is best suited for the synthesis of DNA sequences up to 50-mers in length. |
| 特性： |
| - Chemical Name: 5' CPG Succinyl 2'-O-Acetyl-3'-O-DMT Uridine & 5' CPG Succinyl 3'-O-Acetyl-2'-O-DMT Uridine- Appearance: white powder |
| 用途： |
| - Synthesis conditions: Include a nonsense base as the 3' terminal base for sequence entry systems.- leavage conditions:For cleavage use ammonia/LiCl (15 mg LiCl in 1 mL concentrated aqueous ammonia, ca 0.1N LiCl) or methylamine/ammonia/LiCl for 90 minutes at 25°C. Filter off CPG support and collect supernant.- Deprotection conditions: Deprotect supernant in ammonia/LiCl (15 mg LiCl in 1 mL concentrated aqueous ammonia, ca 0.1N LiCl) for 6 hours at 65°C or methylamine/ammonia/LiCl for about 3-4 hours at 65°C.- Purification and Analysis:If the trityl has previously been removed from the oligo, the lithium salt is removed by precipitation from cold ethanol/salt or using Sephadex G-25 filtration (NAP). Alternatively, the crude TRITYL-ON oligonucleotide can be desalted and purified using commercially available reverse phase oligo purification cartridges (MicroPure II, BTI MP-1602).- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:0 |
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QQ:1749072012