TAMRA-Phos-CPG, 6-Carboxy Single Isomer (5'-DMT-mdC(TEG-TAMRA)-Phos-CPG); 500 Å
货号：BG5-5008B-1,BG5-5008B-100
规格：1 g,100 mg
价格：12663,1899
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Biosearch Technologies' 5'-DMT-mdC(TEG-TAMRA)-Phosphate CPG is based on a modified cytosine residue linked to the TAMRA moiety via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via a phosphate linkage. Our TAMRA CPG supports allow for the introduction of a reporter or quencher molecule onto the 3'-terminus end of the oligonucleotide. |
| 特性： |
| - Formula:C77H91N8O21PS- Molecular Weight: 1527.63- Appearance:pink powder- Absorption Maximum (Lambda Max): 555- Extinction Coefficient at Lambda max:90000- Extinction Coefficient at 260 nm:31980- Fluorescence Maximum: 576 |
| 用途： |
| - Synthesis conditions: Synthesis can be performed on these supports according to standard procedures with deprotection and cleavage modifications noted. The phosphate linkage is susceptible to degradation if the Tamra CPG support is left at room temperature (25°C) over a 24 hour period. The subsequent cleavage may affect the overall yield.- Cleavage conditions:Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia or methylamine must be avoided, as it will cause degradation of the TAMRA moiety.- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 60° C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 60°C. Image of cleaved and deprotected structure: - The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 998.91 |
| 数据： |
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QQ:1749072012