DABCYL-C3-CPG (O-DMT-N-DABCYL-3-aminopropan-1,2-diol-Suc-CPG); 500 Å
货号:BG5-5026-1,BG5-5026-100
规格:1 g,100 mg
价格:11256,1407
产品类型:核酸合成
品牌:LGC Biosearch Technologies
| DABCYL C3 CPG is synthesized with a three carbon linker arm and is attached to the solid support via a succinate linkage. This support allows for 3' labeling of molecular beacons and acts as a quencher moiety. DABCYL is used as a quencher for fluorophore groups such as fluorescein, TAMRA and JOE. DABCYL absorbs between 400 to 525 nm with maximum quenching at 474 nm. |
| 特性: |
| - Chemical Name:DABCYL-C3-CPG (O-DMT-N-DABCYL-3-aminopropan-1,2-diol-Suc-CPG); 500 Å- Formula:C18H20N4O3 - Appearance:orange powder- Absorption Maximum (Lambda Max):474 |
| 用途: |
| - Protecting group deblock conditions:Standard detritylation- Cleavage conditions:This support may be cleaved using concentrated NH4OH or t-butylamine/H2O (TAMRA and other rhodamine-based dyes require deprotection with t-butylamine).- Deprotection conditions:-If you are using fast deprotecting amidites [i.e., C(Ac), G(DMF), G(PAC)], deprotect in concentrated NH4OH for 1 hour at 60 °C.
-If you are using standard amidites [i.e., A(Bz), G(iBu)], deprotect in concentrated NH4OH for 5 hours at 60 °C.
-If you are using base sensitive fluorophores (i.e., TAMRA or other rhodamine-based dyes), we recommend deprotecting oligonucleotides in t-butylamine/H2O for 12 hours at 60 °C.- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:404.36 |
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