Corticosterone Competitive ELISA Kit/皮质酮竞争性ELISA试剂盒

货号：EIACORT,EIACORTX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	18.6 pg/mL
检测范围	78.125-10,000 pg/mL
样本类型/体积	粪便提取物 50 µL 血浆 1 µL 血清 1 µL 上清液 50 µL 尿液 2.5 µL
操作时间	1 hr 20 min
测试时间	1 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	7.9%
批内变异系数（CV）	5.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Corticosterone
Protein aliases	17-Deoxycortisol, Reichstein's substance H, Kendall's compound B, C21H30O4
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Corticosterone ELISA quantitates coritcosterone in serum, plasma, urine, dried fecal extracts or cell culture medium.皮质酮ELISA可定量测定血清，血浆，尿液，干燥的粪便提取物或细胞培养基中的皮质酮。Principle of the method 方法原理The Corticosterone Competitive ELISA research-use-only kit is designed to quantitatively measure corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.皮质类固醇竞争性ELISA试剂盒用于定量测量血清，血浆，尿液，提取的干燥粪便样本和组织培养基样本中存在的皮质酮。该试剂盒可测量血清，血浆和粪便提取物中的总皮质酮含量。提供皮质类固醇原液以生成测定的标准曲线，所有样品均应从标准曲线上读出。请选择最适合您样品浓度的标准范围。将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获羊抗体。将皮质酮-过氧化物酶结合物添加到孔中的标准品和样品中。通过向每个孔添加针对皮质酮的多克隆抗体来启动结合反应。温育1小时后，洗涤板并加入底物。底物与结合的皮质酮-过氧化物酶缀合物反应。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CORTICOSTERONE is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].皮质酮是一种肾上腺皮质类固醇，具有适度但显着的盐皮质激素和糖皮质激素活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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