Clusterin Human ELISA Kit/人簇蛋白ELISA试剂盒
货号：EHCLU,EHCLUX5,EHCLUX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 19.53-20,000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Apolipoprotein J |
| Protein aliases | Aging-associated gene 4 protein, aging-associated protein 4, Apo-J, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, Clusterin, Clusterin alpha chain, Clusterin beta chain, Complement cytolysis inhibitor, Complement cytolysis inhibitor a chain, Complement cytolysis inhibitor b chain, complement lysis inhibitor, Complement-associated protein SP-40, 40, Ku70-binding protein 1, NA1/NA2, SGP-2, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2, TRPM-2 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AAG4, APO-J, APOJ, CLI, CLU, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2 |
| Gene ID | (Human) 1191 |
| Gene symbol | CLU |
| UniProt ID | (Human) P10909 |
| The Human Clusterin ELISA quantitates Hu Clusterin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Clusterin.人ClusterinELISA可以定量人类血清，血浆或细胞培养基中的人Clusterin。 该检测试剂盒将专门识别天然和重组的Clusterin。Principle of the method 方法原理The Human Clusterin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Clusterin固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.异构体1充当阻止非天然蛋白聚集的细胞外伴侣。防止压力诱导的血浆蛋白聚集。抑制APP，APOC2，B2M，CALCA，CSN3，SNCA和易于聚集的LYZ变体（体外）形成淀粉样蛋白原纤维。不需要ATP。将部分展开的蛋白质保持在适合随后被其他伴侣（例如HSPA8 / HSC70）重折叠的状态。本身不会折叠蛋白质。与细胞表面受体的结合触发伴侣-客户复合物的内在化和随后的溶酶体或蛋白酶体降解。分泌的同工型1通过补体保护细胞免于凋亡和细胞溶解。细胞内同工型与遍在蛋白和SCF（SKP1-CUL1-F-box蛋白）E3遍在蛋白-蛋白连接酶复合物相互作用，并促进遍在蛋白化和随后蛋白酶体降解靶蛋白。促进COMMD1和IKBKB的蛋白酶体降解。调节NF-κB的转录活性。核亚型促进细胞凋亡。线粒体同工型抑制BAX依赖的细胞色素c释放到细胞质中并抑制细胞凋亡。在细胞增殖的调节中起作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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