uPAR (PLAUR) Human ELISA Kit/人uPAR (PLAUR) ELISA试剂盒
货号：EHPLAUR,EHPLAURX5,EHPLAURX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 16.38-4000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | uPAR (CD87) |
| Protein aliases | CD87, Monocyte activation antigen Mo3, U-PAR, uPAR, u-plasminogen activator receptor form 2, Urokinase plasminogen activator surface receptor, urokinase-type plasminogen activator (uPA) receptor |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CD87, MO3, PLAUR, U-PAR, UPAR, URKR |
| Gene ID | (Human) 5329 |
| Gene symbol | PLAUR |
| UniProt ID | (Human) Q03405 |
| The Human uPAR (PLAUR) ELISA quantitates Hu uPAR in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu uPAR.人uPAR（PLAUR）ELISA定量了人血清，血浆或细胞培养基中的人uPAR。 该检测试剂盒将专门识别天然和重组人uPAR。Principle of the method 方法原理The Human uPAR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人uPAR固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The urokinase-type plasminogen activator receptor is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes. The human PLAUR cDNA encodes 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. It produces a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. PLAUR, also known as UPAR, is directly associated with the carbohydrate-binding domain of SELL in the membrane of neutrophils, an association analogous to that between PLAUR and beta-2 integrins. PLAUR-mediated calcium mobilization is SELL dependent. UPAR mRNA levels correlate with the invasive potential of endometrial carcinomas and show a 33-fold increase in UPAR mRNA levels in advanced clinical stage endometrial tumors compared with normal endometrial tissue. Furthermore, the increase in UPAR mRNA levels correlated linearly with the progression of disease stage. UPAR protein expressioin correlated positively with rate of recurrence and mortality in patients with endometrial cancer. UPAR appears to be a useful prognostic marker for advanced endometrial cancer.尿激酶型纤溶酶原激活剂受体是调节细胞表面纤溶酶原激活的关键分子，在许多正常以及病理过程中都起着重要作用。人PLAUR cDNA编码335个氨基酸，其中包括22个残基的预测信号肽和疏水性C端部分。它在单核细胞中产生约50 kD的高度糖基化蛋白，并通过糖基磷脂酰肌醇键合锚定在质膜上。 PLAUR，也称为UPAR，直接与嗜中性粒细胞膜中SELL的糖结合结构域相关，类似于PLAUR和β-2整联蛋白之间的关联。 PLAUR介导的钙动员依赖于SELL。与正常子宫内膜组织相比，UPAR mRNA水平与子宫内膜癌的浸润潜能相关，并且在晚期临床子宫内膜肿瘤中显示UPAR mRNA水平增加了33倍。此外，UPAR mRNA水平的增加与疾病阶段的进展呈线性相关。 UPAR蛋白表达与子宫内膜癌患者的复发率和死亡率呈正相关。 UPAR似乎是晚期子宫内膜癌的有用预后指标。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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