Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit/心钠素竞争性ELISA试剂盒

货号：EIAANP

规格：96 tests

价格：7238

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.25 ng/mL
检测范围	0.741-180 ng/mL
样本类型/体积	血浆 50 µL 上清液 50 µL 尿液 12.5 µL
操作时间	1 hr 20 min
测试时间	1 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	7.6%
批内变异系数（CV）	6.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Atrial Natriuretic Peptide
Protein aliases	ANP
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma, urine or cell culture medium.心钠素竞争性ELISA定量测定人血浆，尿液或细胞培养基中的ANP。Principle of the method 方法原理The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma, urine, and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.心钠素竞争性ELISA试剂盒用于定量测量血浆，尿液和组织培养基样品中存在的ANP。 不建议将该试剂盒用于未提取的血清或血浆样品。 提供了ANP标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。 将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获兔抗体。 将ANP-过氧化物酶结合物添加到孔中的标准品和样品中。 通过向每个孔中添加针对ANP的抗体来启动结合反应。 温育1小时后，洗涤板并加入底物。 底物与结合的ANP-过氧化物酶缀合物反应。 短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息ANP is derived from the 151 amino acid long protein preproANP3. The prepro protein is cleaved to generate proANP. ANP is derived from amino acids 99-126 to form the 28 amino acid peptide with a disulfide bond between amino acids 7 and 23. ANP is the predominant member of a family of structurally and functionally related peptide hormones that exert a wide array of effects on cardiovascular and renal function. ANP is identical in a large number of species. Human, pig, sheep, and dog ANP have identical sequences and this kit will measure samples from these species identically. Rat and mouse ANP molecules differ at amino acid 12 but show complete cross reactivity with this kit.ANP衍生自151个氨基酸长的蛋白preproANP3。 prepro蛋白被切割产生proANP。 ANP源自99-126位氨基酸，形成28个氨基酸的肽，在7位和23位氨基酸之间具有二硫键。ANP是结构和功能相关的肽激素家族的主要成员，可对多种激素产生广泛影响 心血管和肾功能。 ANP在许多物种中都是相同的。 人，猪，绵羊和狗的ANP具有相同的序列，该试剂盒将对这些物种的样品进行相同的测量。 大鼠和小鼠的ANP分子在第12个氨基酸处不同，但与该试剂盒显示完全的交叉反应性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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