| The Rat Platelet-Derived Growth Factor AA (Rt PDGF AA) ELISA quantitates Rt PDGF AA in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt PDGF AA.大鼠血小板衍生生长因子AA（大鼠PDGF AA）ELISA定量了大鼠血清，血浆或细胞培养基中的大鼠PDGF AA。 该检测试剂盒将专门识别天然和重组大鼠PDGF AA。Principle of the method 方法原理The Rat PDGF AA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠PDGF AA固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.血小板衍生的生长因子（PDGF）是血清中存在但血浆中不存在的主要促有丝分裂因子。发现它是从血液凝结形成血清过程中活化的血小板的α颗粒分泌的。随后的研究表明，PDGF不是一个分子，而是三个分子，每个都是两个截然不同但结构上相关的肽链（称为A和B）的二聚体组合。二聚体同种型PDGF-AA，AB和BB在各种细胞类型及其作用中差异表达通过两种截然不同的受体（称为α和β）介导的β-内酰胺酶。同种型与每个受体的结合存在差异。通常，PDGF亚型是结缔组织细胞的有效促细胞分裂剂，包括真皮成纤维细胞，神经胶质细胞，动脉平滑肌细胞以及某些上皮和内皮细胞。除了作为有丝分裂原的活性外，PDGF还具有成纤维细胞，平滑肌细胞，嗜中性粒细胞和单核细胞的趋化作用。 PDGF的其他报道的活性包括中性粒细胞和单核细胞刺激颗粒释放，莱伊迪格细胞促进类固醇合成，刺激中性粒细胞吞噬作用，抑制自然杀伤（NK）细胞活性，刺激胶原蛋白合成，调节血小板反应蛋白的表达和分泌，刺激胶原酶的活性和分泌，体外诱导大鼠主动脉条的收缩，以及瞬时诱导T细胞IL-2分泌，同时下调IL-4和IFN-γ的产生，这些暂时的作用可能会导致克隆扩增分化之前先进行抗原活化的B和T辅助淋巴细胞。 PDGF在整个CNS的神经元中也普遍存在，在神经元的存活和再生以及胶质细胞增殖和分化的介导中起重要作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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