CAL Fluor Red 610 CPG (5'-DMT-T(C6-CAL Fluor Red 610)-Glyc-CPG); 500 Å
货号：BG5-5082-1,BG5-5082-100
规格：1 g,100 mg
价格：21105,2673.3
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Cal Fluor Red 610 CPG fluoresces in the orange-red region of the visible spectrum. Cal Fluor Red 610 CPG support allows for the introduction of the Cal Fluor Red 610 moiety onto the 3'-terminus of the oligonucleotide. Biosearch Technologies has developed the 5'DMT protected Cal Fluor Red 610 CPG 3'-Glycolate support which is specifically suited for the preparation of dual labeled Fluorescent Energy Transfer probes. BHQ-2 will quench the Cal Fluor Red 610 moiety. Cal FLuor Red 610 is an alternative for Texas Red and ROX. |
| 特性： |
| - Appearance:purple powder- Absorption Maximum (Lambda Max):593- Extinction Coefficient at Lambda max:99300- Extinction Coefficient at 260 nm:27300- Fluorescence Maximum:610 |
| 用途： |
Synthesis conditions: Due to the mild deprotection conditions we recommend using fast deprotecting amidites with this support.Cleavage conditions:Deprotection conditions: Cleave and deprotect the CAL dye labeled oligo in 2-Methoxyethylamine (Ald# 241067-50) and MeOH (1:3) for 6 hours at 25°C or 3 hours at 60 °C.
The use of strong bases, such as concentrated ammonia, causes degradation of dye and should be avoided.CAL Fluor dyes are light sensitive, prolonged exposure to light causes photobleaching.Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:1171.15 |
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