STAT3 (Total) Human InstantOne ELISA Kit/人STAT3 (Total) InstantOne ELISA试剂盒
货号：85-86101-11
规格：96 tests
价格：6634
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | STAT3 |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 6774 |
| Gene symbol | STAT3 |
Gene aliases | ADMIO, ADMIO1, APRF, HIES |
UniProt ID | (Human) P40763 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of total human STAT3 in cell lysates. Detection of mouse and rat STAT3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解液中总人STAT3的。 预期检测到小鼠和大鼠STAT3。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.STAT3属于STAT（信号转导和转录激活物）蛋白家族，该蛋白被受体相关激酶磷酸化，易位至细胞核，并作为响应细胞因子和生长因子的转录因子。 STAT3的转录激活需要诸如CREB结合蛋白之类的共激活剂。 STAT3也可以被α-干扰素，γ-干扰素，EGF，PDGF和IL6激活。 JAK1和JAK2在酪氨酸705上的磷酸化对于STAT3二聚体形成，核易位和DNA结合活性至关重要。在人类中，STAT3基因位于17号染色体的q臂上。已证明STAT3被IFN-α激活，但未被IFN-β激活。与STAT3相关的转录因子是c-Jun和环状AMP响应增强子结合蛋白（CREB）。 STAT3介导响应细胞刺激的多种基因的表达，因此在许多细胞过程（例如细胞生长和凋亡）中起着关键作用。小型GTPase Rac1已显示出结合并调节STAT3的活性，而PIAS3蛋白是STAT3的特异性抑制剂。已经描述了三种交替剪接的转录物变体，它们编码STAT3的不同同工型。敲除小鼠中的STAT3基因在胚胎早期阶段是致命的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| STAT3 (Total) Human InstantOne™ ELISA KitRepresentative data for STAT3 (Total) Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the bands around 89 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.STAT3（总）多物种InstantOne™ELISA的代表性数据。 A431细胞用10 µM AG1478（EGFR激酶抑制剂）进行预处理，洗涤后不刺激（-），或用10 ng / mL EGF刺激10分钟（+）。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的89 kDa附近条带的强度与InstantOne™ELISA吸收值相关。 |
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