AKT Multispecies InstantOne ELISA Kit/多物种AKT InstantOne ELISA试剂盒
货号：85-86049-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | AKT Pan |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 10000,(Human) 207,(Human) 208 |
| Gene symbol | AKT3, AKT1, AKT2 |
Gene aliases | MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA |
UniProt ID | (Human) Q9Y243,(Human) P31749,(Human) P31751 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Thr308 and Ser473) in cell lysates. Detection of phosphorylated rat AKT 1/2/3 (Thr308 and Ser473) and total rat AKT 1 is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于准确测量细胞裂解液中人，小鼠和仓鼠的总AKT 1和磷酸化的AKT 1/2/3（Thr308和Ser473）的试剂盒。 还期望检测到磷酸化的大鼠AKT 1/2/3（Thr308和Ser473）和总大鼠AKT 1。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息AKT (protein kinase B, PKB, RAS-alpha) is a 57kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. AKT is activated by insulin, PI3K, IGF1 and various other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. The AKT pathway is a major target for cancer therpeutics where AKT signaling dysfunction has implicated in many types of cancer, including cancer syndromes known as phakomatoses.AKT（蛋白激酶B，PKB，RAS-α）是一种57kDa的丝氨酸/苏氨酸激酶，在多种生物反应中发挥重要作用，例如通过磷酸化多种蛋白来调节代谢，细胞存活和生长。 AKT被胰岛素，PI3K，IGF1和各种其他生长与生存因子激活。 AKT通过抑制磷酸化和使包括叉头转录因子和caspase-9在内的多个靶失活来抑制细胞凋亡，从而促进细胞存活。 Akt具有三种哺乳动物同工型：AKT1（PKB alpha），AKT2（PKB beta）和AKT3（PKB gamma），其中AKT2和AKT3与AKT1同工型约有82％相同。每个同工型具有一个pleckstrin同源性（PH）域，一个激酶域和一个羧基末端调节域。 AKT最初是从逆转录病毒AKT8中克隆的，是许多信号转导途径的关键调节因子。 AKT途径是癌症治疗学的主要目标，其中AKT信号功能障碍与许多类型的癌症有关，包括被称为白眼的癌症综合征。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| AKT Multispecies InstantOne™ ELISA KitRepresentative data for AKT Multispecies InstantOne™ ELISA. MCF7 cells were treated with 10 µM wortmannin for 60 min (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cell lysates were analyzed for levels of Total AKT 1 (left), phospho-AKT 1/2/3 (Ser473) (center), and phospho AKT 1/2/3 (Thr308) (right) using the AKT Activation InstantOne™ ELISA.AKT Multispecies InstantOne™ELISA的代表性数据。 MCF7细胞用10 µM渥曼青霉素处理60 min（-）或用10 ng/mL胰岛素刺激10 min（+）。使用AKT Activation InstantOne™ELISA分析细胞裂解液的总AKT 1（左），磷酸-AKT 1/2/3（Ser473）（中）和磷酸AKT 1/2/3（Thr308）（右）的水平 。 |
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