TAMRA Amidite (N-TAMRA-Piperidinyl), 5,6-Carboxy
货号：BNS-5027-100,BNS-5027-250,BNS-5027-50
规格：100 µmol,250 mg,50 µmol
价格：2110.5,8442,1195.95
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| TAMRA Amidite is used for the labeling of oligonucleotides and fluorogenic probes. This product is prepared using the 5- and 6- carboxytetramethylrhodamine isomers and can only be added to the 5' terminus of the oligo or internally to free hydroxyls. |
| 特性： |
| - Chemical Name:1-[(Tetramethylrhodamin-5(6)-yl)carbonyl]piperidin-4-yl-2-cyanoethyl-N,N-diisopropyl phosphoramidite- Formula:C39H48N5O6P- Molecular Weight:713.33- Appearance:pink solid- Absorption Maximum (Lambda Max):555- Extinction Coefficient at Lambda max:90000- Extinction Coefficient at 260 nm:31980- Fluorescence Maximum:576 |
| 用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved. Product should only be used for DNA synthesis with standard reagents ( i.e. Ethylthio Tetrazole, 0.02M Iodoine/THF).Dilution:100 µmol/mLCoupling:10-minute couplingDeprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 60 °C. Strong bases, such as concentrated ammonia cause degradation of rhodamine dyes.Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:575.56 |
| 数据： |
| |
QQ:1749072012