TAMRA Amidite (N-TAMRA-Piperidinyl), 5,6-Carboxy
货号：BNS-5027-100,BNS-5027-250,BNS-5027-50
规格：100 µmol,250 mg,50 µmol
价格：2110.5,8442,1195.95
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| TAMRA Amidite is used for the labeling of oligonucleotides and fluorogenic probes. This product is prepared using the 5- and 6- carboxytetramethylrhodamine isomers and can only be added to the 5' terminus of the oligo or internally to free hydroxyls. |
| 特性： |
| - Chemical Name:1-[(Tetramethylrhodamin-5(6)-yl)carbonyl]piperidin-4-yl-2-cyanoethyl-N,N-diisopropyl phosphoramidite- Formula:C39H48N5O6P- Molecular Weight:713.33- Appearance:pink solid- Absorption Maximum (Lambda Max):555- Extinction Coefficient at Lambda max:90000- Extinction Coefficient at 260 nm:31980- Fluorescence Maximum:576 |
| 用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved. Product should only be used for DNA synthesis with standard reagents ( i.e. Ethylthio Tetrazole, 0.02M Iodoine/THF).Dilution:100 µmol/mLCoupling:10-minute couplingDeprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 60 °C. Strong bases, such as concentrated ammonia cause degradation of rhodamine dyes.Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:575.56 |
| 数据： |
| |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://www.biorod.cn/thread-20721.htm
QQ:1749072012