IL-37 Human Uncoated ELISA Kit with Plates/人IL-37 ELISA试剂盒含未包被板
货号:88-52103-22
规格:2 x 96 tests
价格:3125
产品类型:自定义ELISA
品牌:Thermo Fisher
物种:人
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | 31.3 pg/mL |
检测范围 | 31.3-2,000 pg/mL |
样本类型/体积 | 血清 50 μL 血浆 50 μL 上清液 50 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体:Pre-titrated, 纯化抗体 - 检测抗体:Pre-titrated, biotin偶联抗体 - 标准品:重组细胞因子,用于生成标准曲线和校准样品 - 10X包被缓冲液:用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液:封闭和稀释检测抗体和酶的缓冲液 - 酶:Pre-titrated Avidin-HRP - 底物:1X TMB溶液 - 分析证书:特定批次说明书用于抗体和标准品稀释 - 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-76,-86) |
储存温度 | 2-8℃ |
Protein name | IL-37 |
Protein aliases | FIL1 zeta, IL-1 zeta, IL-1F7, IL-1F7b (IL-1H4, IL-1H, IL-1RP1), IL-1RP1, IL-1X, IL-1X protein, IL-37, IL1F7 (canonical product IL-1F7b), interleukin 1 family member 7, interleukin 1, zeta, Interleukin-1 family member 7, Interleukin-1 homolog 4, interleukin-1 superfamily z, Interleukin-1 zeta, Interleukin-1-related protein, Interleukin-23, Interleukin-37 |
物种(Tested) | 人 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene aliases | FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-37, IL1F7, IL1H4, IL1RP1, IL37 |
Gene ID | (Human) 27178 |
Gene symbol | IL37 |
UniProt ID | (Human) Q9NZH6 |
The Human Interleukin 37 (IL-37) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-37. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素37(IL-37)未包被ELISA试剂盒包含预先匹配的抗体对,板和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量人IL-37的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported.该基因编码的蛋白质是白介素1细胞因子家族的成员。 该细胞因子可以与白介素18受体(IL18R1 / IL-1Rrp)结合,也可以是其配体。 该细胞因子还与白介素18结合蛋白(IL18BP)结合,白介素18的抑制性结合蛋白(IL18),随后与IL18受体β亚基形成复合物,并通过该复合物抑制IL18的活性。 该基因与其他8个白介素1家族基因一起在2号染色体上形成了一个细胞因子基因簇。据报道,已有5个选择性剪接的转录变体编码不同的同工型。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
数据: |
IL-37 Human Uncoated ELISA Kit with PlatesRepresentative standard curve for Human IL-37 ELISA |
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