IL-37 Human Uncoated ELISA Kit with Plates/人IL-37 ELISA试剂盒含未包被板

2024-10-17

IL-37 Human Uncoated ELISA Kit with Plates/人IL-37 ELISA试剂盒含未包被板

货号:88-52103-22

规格:2 x 96 tests

价格:3125

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

31.3 pg/mL

检测范围31.3-2,000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

2X 96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

- 10X包被缓冲液:用于包被捕获抗体的缓冲液

- 5XELISA/ELISPOT稀释液:封闭和稀释检测抗体和酶的缓冲液

- 酶:Pre-titrated Avidin-HRP

- 底物:1X TMB溶液

- 分析证书:特定批次说明书用于抗体和标准品稀释

- 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-76,-86)

储存温度

2-8℃

Protein nameIL-37
Protein aliasesFIL1 zeta, IL-1 zeta, IL-1F7, IL-1F7b (IL-1H4, IL-1H, IL-1RP1), IL-1RP1, IL-1X, IL-1X protein, IL-37, IL1F7 (canonical product IL-1F7b), interleukin 1 family member 7, interleukin 1, zeta, Interleukin-1 family member 7, Interleukin-1 homolog 4, interleukin-1 superfamily z, Interleukin-1 zeta, Interleukin-1-related protein, Interleukin-23, Interleukin-37

物种(Tested)

试剂盒类型

Uncoated ELISA Kitwith Plates

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesFIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-37, IL1F7, IL1H4, IL1RP1, IL37
Gene ID(Human) 27178
Gene symbolIL37
UniProt ID(Human) Q9NZH6
The Human Interleukin 37 (IL-37) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-37. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素37(IL-37)未包被ELISA试剂盒包含预先匹配的抗体对,板和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量人IL-37的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported.该基因编码的蛋白质是白介素1细胞因子家族的成员。 该细胞因子可以与白介素18受体(IL18R1 / IL-1Rrp)结合,也可以是其配体。 该细胞因子还与白介素18结合蛋白(IL18BP)结合,白介素18的抑制性结合蛋白(IL18),随后与IL18受体β亚基形成复合物,并通过该复合物抑制IL18的活性。 该基因与其他8个白介素1家族基因一起在2号染色体上形成了一个细胞因子基因簇。据报道,已有5个选择性剪接的转录变体编码不同的同工型。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-37 Human Uncoated ELISA Kit with PlatesRepresentative standard curve for Human IL-37 ELISA
技术参数
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