BHQ-1 Amidite
货号：BNS-5051N-50,BNS-5051N-100,BNS-5051N-250
规格：50 mg,100 mg,250 mg
价格：2251.2,4221,9849
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ-1 amidite is used for the 5' labeling of fluorogenic probes. This amidite does not contain a DMT protecting group. The BHQ-1 quencher was designed to have maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range. |
| 特性： |
| - Chemical Name:4'-(2-Nitro-4-toluyldiazo)-2'-methoxy-5'-methyl-azobenzene-4''-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite- Formula:C34H45N8O5P- Molecular Weight:676.75- Appearance:dark red solid- Absorption Maximum (Lambda Max):534- Extinction Coefficient at Lambda max:34000 - Extinction Coefficient at 260 nm:8000 |
| 产品用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved.Dilution:100 µmol/mLDeprotection conditions:Deprotection conditions are dependent upon the type of amidites used for the synthesis of the oligo. If using standard amidites deprotect in concentrated NH4OH for 5 hours at 60 °C. If using fast deprotecting amidites deprotect in concentrated NH4OH for 1 hour at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA or Rhodamine based dyes) we recommend deprotecting the dye labeled oligonucleotide in t-butylamine/H2O (1:3) for 6 hours at 60 °C.If all the solution is not used during the synthesis, it can be stored under argon and its functionality maintained up to 48 hours.
BHQ-1 amidite is susceptible to oxidation when left out in the open or undessicated.
For detailed information on BHQ fragmentation by MALDI-TOF MS, refer to your BHQ Licensee's Manual Appendix V. |
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QQ:1749072012