ROX Synthesis Column (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å
货号：CG5-5021-1,CG5-5021-2,CG5-5021-5
规格：1 µmol,200 nmol,50 nmol
价格：1266.3,351.75,281.4
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| This column, packed with Biosearch Technologies' 5'-DMT-mdC(TEG-ROX)-Phosphate CPG, allows for the introduction of a reporter molecule onto the 3'-terminus end of the oligonucleotide. 5'-DMT-mdC(TEG-ROX)-Phosphate CPG is based on a modified cytosine residue linked to the ROX moiety via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via phosphate linkage. |
| 特性： |
| ▪ Appearance：purple powder▪ Absorption Maximum (Lambda Max)：575▪ Extinction Coefficient at Lambda max：91000▪ Extinction Coefficient at 260 nm：38500▪ Fluorescence Maximum：602 |
| 产品用途： |
| Synthesis conditions:Follow the instrument manufacturer's protocol, with noted changes, when using this product. Synthesis can be performed on these supports according to standard procedures with deprotection and cleavage modifications noted.Cleavage conditions:Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia must be avoided, as it will cause degradation of the ROX moiety.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 65° C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 65°C. The use of ammonia must be avoided, as it will cause degradation of the ROX moiety. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：1103.06 |
| 数据： |
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