| The ProQuantum HumanMIP-1 betaImmunoassay Kit is designed to provide highly sensitive quantitative measurements of human MIP-1 beta protein in small sample volumes. Utilizing proximity ligation assay (PLA) technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.ProQuantum 免疫检测试剂盒进行少量人MIP-1 beta蛋白的高灵敏度定量检测。 利用邻近连接测定（PLA）技术，该测定将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。 友好的工作流程与直观的基于云的分析软件相结合，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定法使用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。 在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后创建模板链进行扩增。 该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和宽动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。 每个试剂盒中总共有7个组件。 首先，混合抗体-结合物物，稀释样品，并在工作板上创建标准曲线。 然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。 合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。 运行完成后，将结果文件导入https://apps.thermofisher.com/apps/proquantum的基于ProQuantum云的软件中。 使用此软件，可以轻松分析数据以获得蛋白质浓度值。 该软件允许您设置标准曲线，设计板布局，设置定制化验说明并获得可靠的逐组统计比较。Target information 靶标信息CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.CCL4（巨噬细胞炎性蛋白1-beta，MIP1B）属于分泌间beta（趋化因子CC）家族。在功能上，CCL4参与趋化和促炎作用以及体内平衡。细菌内毒素刺激巨噬细胞产生CCL4。 CCL4在炎症部位募集并刺激各种炎症细胞。 CCL4由淋巴细胞，巨噬细胞和树突状细胞产生。 CCL4和相关蛋白CCL3均通过调节炎症细胞选定亚组的运输和激活状态，参与宿主对入侵的细菌，病毒，寄生虫和真菌病原体的反应。尽管CCL4和CCL3对单核细胞的作用相似，但它们对淋巴细胞的作用却有所不同，CCL4选择性吸引CD4 +淋巴细胞，而CCL3选择性吸引CD8 +淋巴细胞。另外，已显示两者都是对B细胞，嗜酸性粒细胞和树突状细胞的有效趋化剂。 CCL4的加工形式可以诱导趋化因子受体CCR5的表面表达下调，从而抑制TCR中的CCR5介导的HIV-1进入。 CCL4与CCR5受体具有高亲和力。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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