Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5/山羊抗大鼠 IgG (H+L) 交叉吸附二抗, Cyanine5

2024-10-17

Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5/山羊抗大鼠 IgG (H+L) 交叉吸附二抗, Cyanine5

货号:A10525

规格:1mg

价格:2245

产品类型:荧光二抗

品牌:Thermo Fisher

物种:大鼠

宿主:山羊

抗体亚型:IgG

荧光染料:其它

类型:多抗同型对照:IgG
浓度:2 mg/mL用法:4 µg/mL(ICC);4 µg/mL(IF)
靶标信息抗大鼠第二抗体是具有亲和力的抗体,对大鼠免疫球蛋白具有良好的特异性,可用于检测,分选或纯化其特定靶标。 二抗提供了更多的多功能性,使用户能够使用许多检测系统(例如HRP,AP,荧光)。 它们还可以通过信号放大来提供更高的灵敏度,因为多个二级抗体可以与单个一级抗体结合。 最常见地,通过用来自靶物种的免疫球蛋白的汇集群体对宿主动物进行免疫来产生二抗,并且可以进一步纯化和修饰(即免疫亲和层析,抗体片段化,标记缀合等)以产生高度特异性的试剂。

数据

大鼠IF中的IgG(H + L)交叉吸附二抗(A10525)山羊抗大鼠IgG(H + L)二抗的免疫荧光分析是使用用α微管蛋白(YL1 / 2)大鼠单克隆抗体(产品编号MA1-80017)染色的A549细胞进行的。细胞用4%多聚甲醛固定10分钟,用0.1%Triton™X-100透化10分钟,用1%BSA封闭1小时,并在室温下用2μg/ mL大鼠一抗标记3小时。山羊抗鼠IgG(H + L)二抗Cy5®(产品编号A10525)在含有0.2%BSA的磷酸盐缓冲盐水中以4µg / mL的浓度在室温下使用45分钟,以检测其中的α-微管蛋白。细胞质(面板a:红色)。在SlowFade®Gold Antifade Mountant(产品编号S36938)中,用DAPI将细胞核(面板b:蓝色)染色。 F-肌动蛋白用488 Phalloidin(产品编号A12379),1:300)(面板c:绿色)染色。面板d代表合成图像。单独的二抗(图f)或同种型对照(图e)未观察到非特异性染色。图像以60倍放大率拍摄。

推荐产品:

二抗

荧光二抗

免疫组化一抗

标签抗体

参考文献:

1.TheranosticsSTAT3/NF-κB-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer."A10525 was used in immunohistochemistry to define a novel approach using NF-kappa B-driven suicide gene therapy to treat triple-negative breast cancer"AuthorsKuo WY,Hwu L,Wu CY,Lee JS,Chang CW,Liu RS2.GeneticsIntegration of Orthogonal Signaling by the Notch and Dpp Pathways in Drosophila."A10525 was used in immunohistochemistry to propose that dorsal wing margin enhancer integration of BMP signaling maintains nab expression in proliferating margin descendants"AuthorsStroebele E,Erives A3.NatureImpermanence of dendritic spines in live adult CA1 hippocampus."A10525 was used in immunohistochemistry to test if hippocampal synapse lifetime matches the longevity of hippocampal memory"AuthorsAttardo A,Fitzgerald JE,Schnitzer MJ4.Journal of neuroinflammationIntracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model."A10525 was used in immunohistochemistry - frozen section to develop an in vivo approach using intracerebral grafting of mesenchymal stem cells to secrete interleukin 13"AuthorsLe Blon D,Guglielmetti C,Hoornaert C,Quarta A,Daans J,Dooley D,Lemmens E,Praet J,De Vocht N,Reekmans K,Santermans E,Hens N,Goossens H,Verhoye M,Van der Linden A,Berneman Z,Hendrix S,Ponsaerts P5.AtherosclerosisLineage tracing of cells involved in atherosclerosis."A10525 was used in immunohistochemistry - frozen section to identify the origin of vascular smooth muscle cells and macrophages within atherosclerosis lesions"AuthorsAlbarrán-Juárez J,Kaur H,Grimm M,Offermanns S,Wettschureck N
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