Mouse anti-Human Lambda Light Chain Secondary Antibody/鼠抗人λ 轻链二抗
货号：MA5-11590
规格：500 µL
价格：4025
产品类型：二抗
品牌：Thermo Fisher
抗原：Purified human Lambda light chain, and purified hu
物种：人
宿主：小鼠
抗体亚型：IgG
荧光染料：其它
| 抗体类型: | 二抗 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 应用领域： | 酶联免疫吸附实验
(ELISA) 1 µg/mL 流式细胞分析
(Flow) 1 µg/mL 免疫组化（石蜡）
(IHC (P)) 2-4 µg/mL 免疫印迹
(WB) 1 µg/mL |
| By Western blot, MA5-11590 detects Human Ig lambda light chain at ~25kD in human samples.By ELISA and WB, MA5-11590 does not react with human Ig kappa light chain.靶标信息Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
| 数据： |
| Human Lambda Light Chain Secondary Antibody (MA5-11590) in IHC Formalin-fixed, paraffin-embedded human tonsil stained with Lambda Light Chain antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of plasma cells..Human Lambda Light Chain Secondary Antibody (MA5-11590) in WB Western blot analysis of immunoglobulin (Ig) lambda light chain was performed by loading 25 µg of Raji (IgG/kappa-expressing B cells), Ramos (IgG/lambda-expressing B cells), and Jurkat (T cells) whole cell lysates, 0.25 µg of purified human IgG/kappa and human IgG/lambda, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. Ig lambda light chain was detected at ~25 kD using a mouse anti-human Ig lambda light chain monoclonal antibody (Product # MA5-11590) diluted to a concentration of 0.5 µg/mL in blocking buffer, overnight at 4C overnight on a rocking platform. The blot was washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).Human Lambda Light Chain Secondary Antibody (MA5-11590) in Flow Flow cytometry analysis of surface immunoglobulin (Ig) lambda light chain expression was performed on Ramos (surface and cytoplasmic IgG/lambda-expressing B cells), Raji (cytoplasmic IgG/kappa-expressing B cells), and Jurkat (T cells) cell lines. Cells were stained with a with a mouse anti-human Ig lambda light chain monoclonal antibody (Product # MA5-11590, blue histogram), with a mouse anti-human Ig kappa light chain monoclonal antibody (Product # MA5-12117, red histogram), or mouse IgG1 and mouse IgG2a isotype controls (black histogram), each at a concentration of 1 µg/mL. After incubation of the primary antibody on ice for 1 hour, the cells were stained with a FITC-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31630) at a dilution of 1:500 for 30 minutes on ice. The representative 10,000 cells were obtained for each sample. |
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| 参考文献： |
| 1.Diagnostic pathology Composite lymphoma in the anterior mediastinum: a case report and review of the literature. 2.Archives of dermatology Anetodermic primary cutaneous B-cell lymphoma: a unique clinicopathological presentation of lymphoma possibly associated with antiphospholipid antibodies. |
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