Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 594/山羊抗小鼠IgG2a交叉吸附二抗，Alexa Fluor 594

货号：A-21135

规格：500 µg

价格：3033

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG2a

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 594

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 µg/mL (Flow);1 µg/mL（ICC);1 µg/mL (IF);1-10 µg/mL (IHC)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21135) in IFImmunofluorescence analysis of Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Product # A-21135) was performed using HeLa cells stained with XRCC1 (144) Mouse Monoclonal Antibody (Product # MA1-12640). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL of mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 was used at concentration of 1 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of XRCC1 in the nucleus (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21135) in IFCells were pulsed with BrdU (Product # B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Product # A21300) and green-fluorescent Alexa Fluor® 488 Goat Anti-Mouse IgG1 isotype-specific secondary antibody (Product # A-21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (Product # D1306, D21490). Mitochondria were labeled with anti-COX, Complex IV, subunit I, and red-fluorescent Alexa Fluor® 594 Goat Anti-Mouse IgG2a isotype-specific antibody (Product # A-21135). Coverslips were mounted with Prolong® antifade mounting medium (Product # P-7481). Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21135) in ICCImmunofluorescent analysis of HEC1 (kinetochore, red) in a BHP 2-7 thyroid cancer anaphase cell cultured in complete medium on a glass slide. Cells were fixed with 4% phosphate-buffered paraformaldehyde for 30 min at 37C, permeabilized with 100% ice-cold methanol for 20 min. and blocked with PBS/1% BSA/0.5% Tween 20 at 37C for 30 min. Cells were stained with anti-HEC1, clone 9G3, mouse IgG2a monoclonal antibody (Product # MA1-23308) at a dilution of 1:200 in PBS/1% BSA/0.1% Tween 20 for 60 min at 37C., and then incubated with goat anti-mouse IgG2a AF594 (Product # A-21135) at a dilution of 1:200 for 60 min. at 37C (panel a: red).Alpha tubulin (panel b: green) was stained simultaneously with anti alfa-tubulin, clone DM1A, mouse IgG1 (Product # 14-4502-82) followed by goat anti-mouse IgG1 AF488 (1:200, Cat #A-21121). DNA was stained with Hoechst33342 (panel c: blue). Panels d shows a merged image. Images were taken with a 40x immersion oil objective (for more information: Corver et al., Endocr Relat Cancer. 2018 Jan;25(1):83-97). Data courtesy of Antibody Data Exchange Program.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Scientific reportsInternal epitope tagging informed by relative lack of sequence conservation.2. Nature communicationsCUG-binding protein 1 regulates HSC activation and liver fibrogenesis.3. Nature methodsAn E3-ligase-based method for ablating inhibitory synapses.4. OncotargetLACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis.

技术参数

产品应用 Flow;ICC;IHC;IF

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://www.biorod.cn/thread-21648.htm