Goat anti-Mouse IgM Secondary Antibody, FITC/山羊抗小鼠IgM二抗，FITC

货号：31992

规格：2 mg

价格：4085

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgM

荧光染料：FITC

类型:	二抗	同型对照：
浓度：	1.5 mg/mL	用法：	1:50 - 1:200(Flow)；1:50 - 1:200(ICC)；1:50 - 1:200(IF)；1:50 - 1:200(IHC)；1:50 - 1:200(IP)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31992 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31992 reacts with the heavy chain of mouse IgM, but does not react against IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species.Store product protected from light at 4°C until opened. To extend the shelf-lifeof this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. The concentration of antibody and buffer salts will decrease to one-half of the original after addition of glycerol. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: ~ 3 moles FITC per mole IgG (lot-dependent).Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration). Product is stable for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.Country of Origin: USA靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgM Secondary Antibody (31992) in FlowFlow cytometry analysis of TRA-1-60 was performed on NCCIT cells. NCCIT cells were harvested, fixed and washed with PBS. Cells were incubated with 0.4 µg of TRA-1-60 monoclonal antibody (Product # MA1-023), followed by incubation with FITC-conjugated goat-anti-mouse IgM secondary antibody (Product # 31992), indicated by the shifted peak (blue line). To control for non-specific binding, cells were also incubated with secondary antibody alone (black line). Incubations were performed for 30 mins in the dark and 10,000 cells were analyzed for each sample Mouse IgM Secondary Antibody (31992) in FlowFlow cytometry analysis of TRA-1-81 was performed on NCCIT cells. NCCIT cells were harvested, fixed and washed with PBS. Cells were incubated with 0.4 µg of TRA-1-60 monoclonal antibody (Product # MA1-024), followed by incubation with FITC-conjugated goat-anti-mouse IgM secondary antibody (Product # 31992), indicated by the shifted peak (blue line). To control for non-specific binding, cells were also incubated with secondary antibody alone (black line). Incubations were performed for 30 mins in the dark and 10,000 cells were analyzed for each sample

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