Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680/山羊抗小鼠 IgG (H+L)二抗，DyLight 680
货号：35518
规格：1 mL
价格：2335
产品类型：荧光二抗
品牌：Thermo Fisher
抗原：Purified Mouse IgG, whole molecule
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：DyLight 680
| 抗体类型: | 荧光二抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 1:25 - 1:100（Flow）；4 µg/mL (ICC)；4 µg/mL (IF); 1:50-1:2,000 (IHC); Assay Dependent (IP); 1:5,000-1:20,000(WB) |
产品详细信息Product # 35518 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 35518 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 680 Amax= 682 nm; Emax= 715 nm. Mole Dye/Mole Protein Ratio is lot-dependent.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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| Mouse IgG (H+L) Secondary Antibody (35518) in IFImmunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight® 680 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight® 680 (Product # 35518) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.Mouse IgG (H+L) Secondary Antibody (35518) in IFImmunofluorescent analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody (Product # MA3-063) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody (Product # 35518) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.Mouse IgG (H+L) Secondary Antibody (35518) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.25 µg/mL) and detected using Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680 (Product # 35518) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences). |
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| 参考文献： |
1.The FEBS journal Regulation of NGF-driven neurite outgrowth by Ins(1,4,5)P3 kinase is specifically associated with the two isoenzymes Itpka and Itpkb in a model of PC12 cells.2.Oncogene Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling.3.PLoS genetics Genome-wide DNA methylation analysis of human pancreatic islets from type 2 diabetic and non-diabetic donors identifies candidate genes that influence insulin secretion.4.Biochemical and biophysical research communications Guanine nucleotide-binding protein subunit beta-2-like 1, a new Annexin A7 interacting protein. |
产品应用 Flow;ICC;IHC;IF;IP;WB
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