Rat anti-Mouse IgG (H+L) Secondary Antibody, HRP/大鼠抗小鼠IgG (H+L)二抗，HRP

货号：04-6020

规格：1 mL

价格：2905

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobin

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：LO-MG-7, LO-MG-1-2, and LO-MG3-7

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	1:500-1:1,000（ELISA）； 1:1,000 (WB)；1:500-1:1,000 (IF)

产品详细信息

This monoclonal Rat anti-Mouse IgG cocktail reacts with all subclasses of mouse IgG and exhibits minimal reactivity to mouse IgA, IgM, and light chains. Cross-reactivity has been observed with swine and human IgG.

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Secondary Antibody (04-6020) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Rat anti-Mouse IgG Secondary Antibody, HRP (Product # 04-6020) at dilutions 1:1,000 (Fig. 1). A 22 kDa band corresponding to SOD2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Food chemistryReduction in IgE reactivity of Pacific mackerel parvalbumin by heat treatment.2. Metallomics : integrated biometal scienceStreptococcus pneumoniae requires iron for its viability and expresses two membrane proteins that bind haemoglobin and haem.3.EndocrinologyThyroid hormone, retinoic acid, and testosterone suppress proliferation and induce markers of differentiation in cultured rat sertoli cells.4.Biochemical and biophysical research communicationsKix domain specific Immunoglobulin A can protect from adverse lung and cerebral pathology induced by Plasmodium berghei ANKA.5.Virus researchImmunological equivalence between mouse brain-derived and Vero cell-derived Japanese encephalitis vaccines.

技术参数

产品应用 ELISA;IF;WB

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