Rabbit anti-Mouse IgG2a Recombinant Secondary Antibody/兔抗小鼠IgG2a重组二抗
货号：SA5-10218
规格：100 µg
价格：2732
产品类型：二抗
品牌：Thermo Fisher
抗原：Peptide corresponding to Mouse IgG2a.
物种：小鼠
宿主：兔
抗体亚型：IgG
克隆号：RM219
荧光染料：其它
| 抗体类型: | 二抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 0.005-0.2 µg/mL (ELISA)； 0.5-2 µg/mL (WB) |
产品详细信息This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
| 数据： |
| Mouse IgG2a Secondary Antibody (SA5-10218) in WB Western blot of nonreduced (-) and reduced (+) mouse IgG2a, using 0.5 µg/mL of Rabbit Anti-Mouse IgG2a Secondary Antibody (Product # SA5-10218). This antibody reacts to nonreduced IgG2a (~150 kDa).Mouse IgG2a Secondary Antibody (SA5-10218) in ELISA ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG2a Secondary Antibody (Product # SA5-10218) reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Rabbit Anti-Mouse IgG2a Secondary Antibody (Product # SA5-10218) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Mouse IgG2a Secondary Antibody (SA5-10218) in ELISAA titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of Rabbit Anti-Mouse IgG2a Secondary Antibody (Product # SA5-10218) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody |
推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
QQ:1749072012