F(ab')2-Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP/F(ab')2-山羊抗小鼠IgG (H+L)高交叉吸附二抗，HRP
货号：A24518
规格：500 µg
价格：3012
产品类型：荧光二抗
品牌：美国精骐
抗原：Gamma Immunoglobins Heavy and Light chains
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：HRP
| 抗体类型: | 荧光二抗 | 同型对照： | |
| 浓度： | | 用法： | 1:500-1:20,000 (ELISA)；1:500-1:20,000 (WB)； 1:500-1:20,000 (IHC) |
靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
| 数据： |
| Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A24518) in WBWestern blot analysis of alpha-fetoprotein was performed by loading 10 µg of whole cell extract from H9 ESCs, H9 ESC-derived hepatoblasts, ESC-derived hepatocyte-like cells, HepG2 cells, HepaRG cells, and primary human hepatocytes in reducing conditions and 10 µL Spectra™ Multicolor Broad Range Protein Ladder (Product # 26634) per well onto a Novex Bolt® 4-12% Bis-Tris Plus Gel with Bolt® MOPS running buffer and Bolt® Antioxidant. Proteins were transferred to a PVDF membrane using the iBlot 2 Dry Blotting System (Product # IB21001), and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. alpha-Fetoprotein was detected at 67 kDa using an alpha-fetoprotein monoclonal antibody (Product # MA5-14666) at a concentration of 1 µg/mL in 5% non-fat milk in TBST overnight at 4°C on a rocking platform, followed by a goat anti-mouse secondary antibody (Product # A24518) at a dilution of 1:10,000 for 1 hour at room temperature. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). |
推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
参考文献： |
| 1. Molecular cancer therapeuticsNanoconjugation of PSMA-Targeting Ligands Enhances Perinuclear Localization and Improves Efficacy of Delivered Alpha-Particle Emitters against Tumor Endothelial Analogues. |
QQ:1749072012