F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC/F（ab'）2-山羊抗小鼠IgG（H + L）交叉吸附二抗，FITC
货号：A24525
规格：500 µg
价格：1606
产品类型：荧光二抗
品牌：Thermo Fisher
抗原：Gamma Immunoglobins Heavy and Light chains
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：FITC
| 类型: | 二抗 | 同型对照： | |
| 浓度： | | 用法： | 0.2 µg/mL(ICC)；0.2 µg/mL(IF) |
靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
| 数据 |
| Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A24525) in IFImmunofluorescence analysis of F (ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, FITC conjugate was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. F (ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, FITC conjugate (Product # A24525) was used at a concentration of 0.2/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of Alpha tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
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