Donkey anti-Mouse IgG (H+L) Secondary Antibody, HRP/驴抗小鼠IgG(H + L)二抗,HRP
货号:PA1-28748
规格:100 µg
价格:2311
产品类型:荧光二抗
品牌:Thermo Fisher
抗原:Full length native mouse IgG (purified)
物种:小鼠
宿主:驴
抗体亚型:IgG
荧光染料:HRP
类型: | 二抗 | 同型对照: | |
浓度: | 1 mg/mL | 用法: | 1:20,000-1:80,000(ELISA);1:1000-1:5000(IHC);1:2000-1:20,000(WB) |
产品详细信息PA1-28748 detects Mouse IgG from mouse samples. Minimum cross reactivity with bovine, chicken, goat, guinea pig, hamster, equine, rat, and ovine samples.靶标信息Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.
数据 |
Mouse IgG (H+L) Secondary Antibody (PA1-28748) in WBWestern blot analysis of rat liver and fish ventricle lysates using Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product # MA1-10891). Whole tissue extracts from rat liver (20µg) and Chaenocephalus aceratus and Notothenia coriiceps ventricles (200µg) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Following transfer, membranes were blocked with 5% milk in PBST and probed with Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product # MA1-10891) at 1:2000 dilution followed by donkey anti-mouse IgG secondary antibody (Product # PA1-28748) at 1:5000 dilution and a chemiluminescent substrate. Data courtesy of the Innovators Program. |
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