Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin/驴抗小鼠IgG（H + L）二抗，Biotin
货号：PA1-28627
规格：1 mg
价格：4039
产品类型：荧光二抗
品牌：Thermo Fisher
抗原：mouse IgG whole molecule
物种：小鼠
宿主：驴
抗体亚型：IgG
荧光染料：Biotin
| 类型: | 二抗 | 同型对照： | |
| 浓度： | 2 mg/mL | 用法： | 1:20,000-1:100,000(ELISA)；1:1000-1:5000(IHC (F))；1:1000-1:5000(IHC (P))；1:2,000-1:5,000(WB) |
产品详细信息PA1-28627 detects Mouse IgG from mouse samples.靶标信息Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.
| 数据 |
| Mouse IgG (H+L) Secondary Antibody (PA1-28627) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by using Donkey anti-Mouse IgG Secondary Antibody, Biotin conjugate (Product # PA1-28627) at dilutions 1:2,000 (Fig. 1) and 1:5,000 (Fig. 2). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). |
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