Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 594/山羊抗小鼠IgG (H+L)交叉吸附二抗，DyLight 594
货号：35511
规格：500 µL
价格：2305
产品类型：荧光二抗
品牌：Thermo Fisher
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：其它
| 抗体类型: | 荧光二抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 4 µg/mL (ICC);4 µg/mL (IF);1:50-1:2,000 (IHC);Assay Dependent (IP);1:5,000-1:20,000(WB) |
产品详细信息Product # 35511 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 35511 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by solid-phase adsorbed to ensure minimal cross-reactivity with human, bovine, horse, rabbit, swine, goat, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 594 Amax= 593 nm; Emax= 618 nm. Mole Dye/Mole Protein Ratio is lot-dependent. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
| 数据 |
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (35511) in IFImmunofluorescence analysis of Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 (Product # 35511) was used at a concentration of 4µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
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| 参考文献： |
| 1.Nature methodsHigh-performance probes for light and electron microscopy. 2.Epigenetics The visualization of large organized chromatin domains enriched in the H3K9me2 mark within a single chromosome in a single cell. |
产品应用 ICC;IHC;IF;IP;WB
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