TRAIL (TNFSF10) Mouse ELISA Kit/小鼠TRAIL (TNFSF10) ELISA试剂盒
货号：EMTNFSF10,EMTNFSF10X5,EMTNFSF10X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 2.46-600 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | TRAIL (CD253) |
| Protein aliases | CD253, Protein TRAIL, TNF-related apoptosis inducing ligand, TNF-related apoptosis-inducing ligand, TRAIL/APO2L, Tumor necrosis factor ligand superfamily member 10 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | A330042I21Rik, AI448571, APO-2L, Ly81, TL2, Tnfsf10, Trail |
| Gene ID | (Mouse) 22035 |
| Gene symbol | Tnfsf10 |
| UniProt ID | (Mouse) P50592 |
The Mouse TRAIL (TNFSF10) ELISA quantitates Ms TRAIL in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TRAIL.小鼠TRAIL（TNFSF10）ELISA定量检测小鼠血清，血浆或细胞培养基中的TRAIL。 该检测试剂盒将专门识别天然和重组Ms TRAIL。Principle of the method 方法原理The Mouse TRAIL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠TRAIL固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.TRAIL是一种细胞因子，属于肿瘤坏死因子（TNF）配体家族。 TRAIL优先诱导转化细胞和肿瘤细胞的凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中均以显着水平表达。 TRAIL与TNF受体超家族的几个成员结合，包括TNFRSF10A / TRAILR1，TNFRSF10B / TRAILR2，TNFRSF10C / TRAILR3，TNFRSF10D / TRAILR4，还可能与TNFRSF11B / OPG结合。可以通过与不能诱导凋亡的诱饵受体TNFRSF10C / TRAILR3，TNFRSF10D / TRAILR4和TNFRSF11B / OPG结合来调节该蛋白的活性。已显示TRAIL蛋白与其受体的结合可触发MAPK8 / JNK，caspase 8和caspase 3的激活。TRAIL的受体包括两个含有死亡域的受体，死亡受体4（DR4）和DR5，以及两个诱饵受体DcR1和DcR2，缺少细胞内信号传导死亡域。 TRAIL是一种II型膜蛋白，可在多种人类组织中表达。像TNF和Fas配体一样，TRAIL诱导许多组织和细胞凋亡和NF-kB激活。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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