TNFSF4 (OX40L) Mouse ELISA Kit/小鼠TNFSF4（OX40L）ELISA试剂盒
货号：EMTNFSF4,EMTNFSF4X5,EMTNFSF4X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 32.77-8000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | OX40L (CD252) |
| Protein aliases | atherosclerosis 1, CD252, OX40 ligand, OX40L, tax-transcriptionally activated glycoprotein 1 ligand, Tumor necrosis factor ligand superfamily member 4 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Ath-1, Ath1, CD134L, gp34, OX-40L, Ox40l, Tnfsf4, TXGP1, Txgp1l |
| Gene ID | (Mouse) 22164 |
| Gene symbol | Tnfsf4 |
| UniProt ID | (Mouse) P43488 |
The Mouse TNFSF4 (OX40L) ELISA quantitates Ms TNFSF4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TNFSF4.小鼠TNFSF4（OX40L）ELISA定量检测小鼠血清，血浆或细胞培养基中的TNFSF4。 该检测试剂盒将专门识别天然和重组Ms TNFSF4。Principle of the method 方法原理The Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠TNFSF4固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.肿瘤坏死因子超家族成员TNFSF4是II型膜结合的非共价连接的同源三聚体蛋白。 它在抗原呈递细胞（例如树突状细胞和活化的B细胞）上表达，并在其他细胞（例如血管内皮细胞，肥大细胞和自然杀伤细胞）上表达。 TNFSF4信号特别是通过TNFRSF4受体表达，主要在CD4 + T细胞上表达，但在某些活化的CD8 + T细胞上也表达。 TNFRSF4 / TNFSF4起到共刺激信号的作用，这是抗原呈递细胞与其靶T细胞之间产生有效相互作用所必需的。 它增强细胞增殖和存活，并增加RANTES，IL-2，IL-3和IFNγ的表达。 TNFRSF4 / TNFSF4信号传导在免疫耐受中起重要作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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