IL-4 Monoclonal Antibody (BVD6-24G2), Biotin

2024-10-18

IL-4 Monoclonal Antibody (BVD6-24G2), Biotin

货号:13-7042-85

规格:500μg

价格:3803

产品类型:流式抗体

品牌:eBioscience

物种:小鼠

宿主:大鼠

抗体亚型:IgG

克隆号:BVD6-24G2

荧光染料:Biotin

类型:单抗同型对照:Rat IgG1 kappa Isotype Control (eBRG1), Biotin
浓度:0.5 mg/mL用法:0.5-2 µg/mL(ELISA);Assay Dependent (Flow)
产品详细信息Description: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.Applications Reported: The BVD6-24G2 antibody is used for detection of mouse IL-4 by ELISA.Applications Tested: The BVD6-24G2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-4 (IL-4) in combination with the purified 11B11 (14-7041) antibody for capture and recombinant mouse IL-4 (14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
数据
IL-4 Antibody (13-7042-85) in FlowStaining of mouse splenocytes stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A for 5 hours with Anti-Mouse CD4 PE (Product # 12-0041-82) and intracellularly stained with Anti-Mouse IL-4 FITC.
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参考文献:

1.The Journal of experimental medicineRab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation."14-5931 was used in ELISA to show the role of MKP5 in restraining p38 MAPK-mediated neutrophil oxidant production, thereby preventing LPS-induced vascular injury in mice."AuthorsCarpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C2.Scientific reportsDer f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells."Published figure using IL-4 monoclonal antibody (Product # 13-7042-81) in ELISA"3.Journal of virologyAltered Glycosylation Patterns Increase Immunogenicity of a Subunit Hepatitis C Virus Vaccine, Inducing Neutralizing Antibodies Which Confer Protection in Mice."13-7042 was used in an ELISpot assay to identify a promising broadly protective HCV vaccine candidate that should be considered for further preclinical and clinical development."4.The Journal of experimental medicineRab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation."14-5931 was used in ELISA to show the role of MKP5 in restraining p38 MAPK-mediated neutrophil oxidant production, thereby preventing LPS-induced vascular injury in mice."AuthorsCarpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C5.PLoS geneticsA Natural Variant of the T Cell Receptor-Signaling Molecule Vav1 Reduces Both Effector T Cell Functions and Susceptibility to Neuroinflammation."13-7042 was used in Flow cytometry/Cell sorting to highlight the importance of Vav1 adaptor functions in inflammatory cytokine production by effector T cells and susceptibility to neuroinflammation."AuthorsKassem S,Gaud G,Bernard I,Benamar M,Dejean AS,Liblau R,Fournié GJ,Colacios C,Malissen B,Saoudi A

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