CD8a Monoclonal Antibody (HIT8a), FITC, eBioscience™
货号：11-0089-42
规格：100 Tests
价格：1822
产品类型：流式抗体
品牌：eBioscience
抗原：CD8a
物种：人/小鼠
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：HIT8a
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.5 µg)/test (Flow） |
产品详细信息Description: The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The HIT8a antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT8a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
| 数据 |
| CD8a Antibody (11-0089-42) in Flow Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (left) or CD8a Monoclonal Antibody, FITC (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Oncoimmunology Azacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects.2.Frontiers in immunology MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.3.Nature communications Clinically compliant spatial and temporal imaging of chimeric antigen receptor T-cells.4.PloS one Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation.5.Molecular medicine reports Skewed T-helper (Th)1/2- and Th17/T regulatory‑cell balances in patients with renal cell carcinoma. |
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