CD47 Monoclonal Antibody (2D3), FITC

货号：11-0478-41,11-0478-42

规格：25 Tests,100 Tests

价格：967,2277

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：2D3

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 μL (1 μg)/test(Flow)

产品详细信息Description: The monoclonal antibody 2D3 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.Applications Reported: This 2D3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

数据

CD47 Antibody (11-0478-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD47 FITC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Stem cells internationalAccelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model."Published figure using CD47 monoclonal antibody (Product # 11-0478-42) in Flow Cytometry"2.BloodCD47 functions as a molecular switch for erythrocyte phagocytosis."11-0478 was used in Flow cytometry/Cell sorting to investigate the role of CD47-SIRPα interaction in promoting phagocytosis of apoptotic cells."AuthorsBurger P,Hilarius-Stokman P,de Korte D,van den Berg TK,van Bruggen R

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