CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), FITC

货号：11-0492-81,11-0492-82

规格：50μg,100μg

价格：925,1545

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：R1-2

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

数据

CD49d (Integrin alpha 4) Antibody (11-0492-81) in FlowStaining of BALB/c splenocytes with 0.25 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD49d (Integrin alpha 4) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in immunologyInterleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry"2.Nature communicationsHelminth-induced IL-4 expands bystander memory CD8+T cells for early control of viral infection."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry"3.ImmunityDifferentiation and Protective Capacity of Virus-Specific CD8+T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche."11-0492 was used in Flow cytometry/Cell sorting to elucidate a strategy of immune evasion by mouse norovirus via the induction of a CD8+ T cell program normally reserved for latent pathogens and persistence in an immune-privileged enteric niche."AuthorsTomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ4.OncotargetThe impact of ranitidine on monocyte responses in the context of solid tumors."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry"5.Shock (Augusta, Ga.)Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression."Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 11-0492-82) in Flow Cytometry"

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