CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), FITC

货号：11-0611-81,11-0611-82

规格：50μg,100μg

价格：925,1545

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：2C9.G3

荧光染料：FITC

类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), FITC
浓度：	0.5 mg/mL	用法：	1 μg/test(Flow)

产品详细信息Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

数据

CD61 (Integrin beta 3) Antibody (11-0611-81) in FlowStaining of C57BL/6 bone marrow cells with 0.5 µg of FITC Armenian Hamster IgG Isotype Control (Product # 11-4888-81) (open histogram) or 0.5 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) FITC (filled histogram). Cells in the lymphoid (left) or myeloid (right) gates were used for analysis.

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参考文献：

1.Epigenetics and chromatinEstablishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points."11-0611 was used in Flow cytometry/Cell sorting to demonstrate that megakaryopoietic profiles are established early in haematopoiesis and are present in the majority of the haematopoietic progenitor population."AuthorsHeuston EF,Keller CA,Lichtenberg J,Giardine B,Anderson SM,Hardison RC,Bodine DM2.Cancer cellHoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia."Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry"3.eLifeThe fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes."Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry"4.Nature communicationsReprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors."11-0611 was used in Flow cytometry/Cell sorting to demonstrate how it is possible to reprogram mouse embryonic fibroblasts into engraftable blood progenitors."AuthorsCheng H,Ang HY,A El Farran C,Li P,Fang HT,Liu TM,Kong SL,Chin ML,Ling WY,Lim EK,Li H,Huber T,Loh KM,Loh YH,Lim B5.The Journal of investigative dermatologyβ1 integrins with individually disrupted cytoplasmic NPxY motifs are embryonic lethal but partially active in the epidermis."Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 11-0611-82) in Flow Cytometry"

技术参数

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