Expansion of CD34+ cells from cord blood in

Stemline™ and Stemline™ II compared to other

commercially available, serum-free, HSC media.

Assays were set up in 24-well tissue culture plates

with each medium tested in triplicate. 1 ml of the

appropriate medium was added to each well, and

the plate was inoculated with 10,000 cells/well.

The cells were counted on day 10 and the fold

increase was determined by cells final/cells inital.

In cord blood, the Stemline™ media outperform

the other serum-free HSC media. While Stemline™

already performed better than or equal to the

other HSC media, Stemline™ II exhibited

approximately a 3 fold increase in expansion

over Stemline™.

Stemline demonstrates superior expansion of cord blood hematopoietic stem cells (HSC). To test the ability of Stemline II Hematopoietic Stem Cell Expansion Medium to expand CD34+ HSCs, researchers at Sigma Aldrich and the University of Kentucky designed a bench-scale expansion assay. Cells were seeded into the wells of 24-well tissue culture plates. One milliliter of medium was added to each well with the appropriate cytokines to stimulate growth (100 ng/ml each of TPO, SCF, and G-CSF). Each condition was performed in triplicate and seeded with 10,000 cells per ml in each well. Due to the clinical importance and the donor-to-donor variability typical to the expansion of umbilical cord blood-derived cells, researchers elected to test 15 donors for expansion and surface antigen expression. The cells were counted on day 10 and the fold increase was determined by cellsfinal/cellsinitial. HSCs from cord blood cultured in Stemline and Stemline II demonstrated superior expansion to those grown in other serum-free HSC media.

Stemline demonstrates superior expansion of bone marrow hematopoietic stem cells (HSC). To test the ability of Stemline II Hematopoietic Stem Cell Expansion Medium to expand CD34+ HSCs, researchers at Sigma Aldrich and the University of Kentucky designed a bench-scale expansion assay. Cells were seeded into the wells of 24-well tissue culture plates. One milliliter of medium was added to each well with the appropriate cytokines to stimulate growth (100 ng/ml each of TPO, SCF, and G-CSF). Each condition was performed in triplicate and seeded with 10,000 cells per ml in each well. The cells were counted on day 14 and the fold increase was determined by cellsfinal/cellsinitial. HSCs from bone marrow cultured in Stemline and Stemline II demonstrated superior expansion to those grown in other serum-free HSC media.