| The ProQuantum Human Leptin Immunoassay Kit is designed to provide quantitative measurements of human leptin in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.ProQuantum人瘦素免疫测定试剂盒旨在提供小样品量中人瘦素的定量测量。 该分析利用基于邻近的扩增技术，将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。 用户友好的工作流程与直观的分析软件相结合，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定利用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。 在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后产生模板链进行扩增。 该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和大动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum software (cloud-based version at https://apps.thermofisher.com/apps/proquantum). Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, apply a 5PL weighted algorithm, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。 每个套件中总共有7个组件（图2）。 首先，混合抗体-缀合物，稀释样品，并在工作板上创建标准曲线。 然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。 合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。 运行完成后，将结果文件导入ProQuantum软件（基于云的版本，网址为https://apps.thermofisher.com/apps/proquantum）。 使用此软件，可以轻松分析数据以获得蛋白质浓度值。 该软件可让您设置标准曲线，设计板布局，应用5PL加权算法并获得可靠的逐组统计比较。Target information 靶标信息Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.瘦素是一种167个氨基酸的长蛋白激素，在调节体重，代谢和生殖功能方面具有重要作用。瘦蛋白的质量约为16 kDa，由肥胖（ob）基因编码。瘦素主要由脂肪细胞表达，这与认为体重是体内脂肪的总量有关。胃上皮细胞和胎盘中的细胞也会分泌少量的瘦素。瘦素受体在下丘脑的区域中高表达，已知对调节体重很重要，在T淋巴细胞和血管内皮细胞中也是如此。瘦素对体重的影响通过对下丘脑中枢的影响来介导，该下丘脑中枢控制进食行为和饥饿，体温和能量消耗。瘦素通过下丘脑瘦素受体参与调节食物摄入，能量消耗和肥胖。瘦素促进造血，血管生成，伤口愈合，炎症，免疫反应，影响青春期发育和胎儿生长。研究已经研究了瘦素在肥胖，神经性厌食症，胰岛素抵抗和高血压中的作用。瘦素还具有生热作用并调节脂肪酸氧化酶。瘦蛋白生产中的缺陷可能导致啮齿动物和人类中的严重遗传性肥胖。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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