JMJD2A Recombinant Rabbit Monoclonal Antibody (6H56L3)/JMJD2A重组兔单克隆抗体（6H56L3）
货号：702449
规格：100 µg
价格：4692
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Protein corresponding to human (aa379-aa628)
物种：人
宿主：兔
抗体亚型：IgG
克隆号：6H56L3
荧光染料：其它
| 类型: | 单抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 2.5 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF) |
产品详细信息This antibody is predicted to react with Monkey, Cat, and Horse.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animalorigin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
| 数据： |
JMJD2A Antibody (702449) in ICCFor immunofluorescence analysis, Ntera2 cells were fixed and permeabilized for detection of endogenous KDM4A using Anti- KDM4A Recombinant Rabbit Monoclonal Antibody (Product # 702449, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KDM4A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of KDM4A. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. JMJD2A Antibody (702449) in ChIPEnrichment of endogenous KDM4A protein at specific gene loci using Anti-KDM4A Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-KDM4A Recombinant Rabbit Monoclonal Antibody (Product # 702449, 5 µg) on sheared chromatin from 2 million NTERA-2 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the MYOG, MYOD, GAPDH region used as positive control target gene, and the region of the GAPDH intron, SAT2 satellite repeat used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method. |
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