H3K18ac Polyclonal Antibody/H3K18ac多克隆抗体
货号：PA5-17801
规格：100 µL
价格：4880
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Synthetic acetylated peptide corresponding to resi
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 1:250(ICC)；1:250(IF)；1:800(IHC (P))；1:2000(WB)；1:2,000(Array) |
产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other acetylated histones.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K18ac Antibody (PA5-17801) in ICCImmunofluorescence analysis of Acetyl-Histone H3 (Lys18) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys18) Rabbit Polyclonal Antibody (Product # PA5-17801) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K18ac Antibody (PA5-17801) in ArrayThe specificity of the antibody, Anti-Acetyl-Histone H3 (Lys18) Polyclonal Antibody (Product # PA5-17801, 1:2000 dilution) to Histone H3K18ac peptide was confirmed using MODified™ Histone Peptide Array (Active Motif, 13001). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used as the secondary antibody. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). The dot blot data obtained (lower panel) was analyzed using Array Analyze software as per the manufacturer's instructions (top panel). |
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