H3K27ac Polyclonal Antibody/H3K27ac多克隆抗体
货号：PA5-40096
规格：50 µg
价格：4880
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：KLH-conjugated synthetic peptide corresponding to
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 1.2 mg/mL | 用法： | 1:100(ELISA)；1:500(ICC)；1:500(IF)；1:1000(WB)；1:2,000(Array) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K27ac Antibody (PA5-40096) in IFImmunofluorescence analysis of Acetyl-Histone H3 (Lys27) Polyclonal Antibody was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys27) Polyclonal Antibody (PA5-40096) at 5 µg/mL in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells with relatively lower expression of Acetyl-Histone H3 (Lys27). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K27ac Antibody (PA5-40096) in ELISATo determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27ac antibody (Product # PA5-40096) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000. |
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