H3K9ac Polyclonal Antibody/H3K9ac多克隆抗体
货号：49-1009
规格：44 µg
价格：4809
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Raised against the region of the histone H3 contai
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | Lot-Specific | 用法： | Assay Dependent(ELISA)；1:250(ICC)；1:250(IF)；1:500(WB)；1:2,000(Array) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K9ac Antibody (49-1009) in IFHeLa cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilizes the morphology of the cells and permeabilizes membranes. (A) Cells were labeled with the anti-H3K9ac antibody (diluted 1:200 and incubated for 1 hour at room temperature) followed by a FITC-labeled goat anti-rabbit secondary antibody. (B) Nuclei were stained using the DNA-specific stain DAPI. Both the anti-H3K9ac antibody and DAPI produced clear nuclear staining H3K9ac Antibody (49-1009) in ICCImmunofluorescence analysis of Acetyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody (Product # 49-1009) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells showing no expression of Acetyl-Histone H3 (Lys9). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1. International journal of molecular sciencesNew Insights into the Role of Polybromo-1 in Prostate Cancer.2. OncotargetIntegrated analysis of the molecular action of Vorinostat identifies epi-sensitised targets for combination therapy. |
QQ:1749072012