H3K17me2aK18ac Polyclonal Antibody/H3K17me2aK18ac多克隆抗体
货号：PA5-40094
规格：50 µg
价格：4880
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：KLH-conjugated synthetic peptide corresponding to
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 0.94 mg/mL | 用法： | 1 µg(ChIP)；1:28,800(ELISA)；1:500(ICC)；1:500(IF)；1:1000(WB)；1:20,000(Array)；Assay dependant(ChIP-Seq) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K17me2aK18ac Antibody (PA5-40094) in IFImmunofluorescent detection of Di-Methy-Acetyl-Histone H3 (Arg17 + Lys18) in NIH3T3 cells using a Di-Methy-Acetyl-Histone H3 (Arg17 + Lys18) polyclonal antibody (Product # PA5-40094). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3R17me2 (asym)K18ac antibody (left) at a dilution of 1:500 in blocking solution followed by detection using an anti-rabbit antibody conjugated to AlexaFluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. H3K17me2aK18ac Antibody (PA5-40094) in ChIPChIP assays were performed on human HeLa cells using a Di-Methy-Acetyl-Histone H3 (Arg17 + Lys18) polyclonal antibody (Product # PA5-40094) and optimized PCR primer pairs for qPCR. ChIP was performed with sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
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