H3K4me2 Polyclonal Antibody/H3K4me2多克隆抗体
货号：PA5-31912
规格：100 µL
价格：4880
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Synthetic peptide corresponding to a region within
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 0.52 mg/mL | 用法： | 1:100-1:1,000(ICC)；1:100-1:1,000(IF)；1:100-1:1,000(IHC (P))；1:500-1:1000(IP)；1:1,000-1:10,000(WB) |
产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K4me2 Antibody (PA5-31912) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys4)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # PA5-31912) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K4me2 Antibody (PA5-31912) in ICCImmunocytochemistry-Immunofluorescence analysis of Di-Methyl-Histone-H3-(Lys4) was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Di-Methyl-Histone H3 (Lys4) Polyclonal Antibody (Product # PA5-31912) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. |
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